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Transcriptomic and peptidomic analysis of protein hydrolysates from the white shrimp (L. vannamei).

TitreTranscriptomic and peptidomic analysis of protein hydrolysates from the white shrimp (L. vannamei).
Type de publicationJournal Article
Year of Publication2014
AuteursRobert, M, Zatylny-Gaudin, C, Fournier, V, Corre, E, Le Corguille, G, Bernay, B, Henry, J
JournalJ Biotechnol
Volume186
Pagination30-7
Date Published2014 Sep 30
ISSN1873-4863
Résumé

An RNAseq approach associated to mass spectrometry was conducted to assess the composition, molecular mass distribution and primary sequence of hydrolytic peptides issued from hydrolysates of white shrimp (Litopenaeus vannamei) by-products. High performance size exclusion chromatography (HPSEC) analyses indicated that 69.2% of the 214-nm-absorbing components had apparent molecular masses below 1000 Da, and 88.3% below 2000 Da. OFFGEL-nLC-MALDI-TOF/TOF and nLC-ESI-MS/MS analyses led to the identification of 808 peptides based on the NCBI EST databank (161,397 entries) completed by the new L. vannamei databank (58,508 entries) that we created from the RNAs of tissues used for hydrolysate production. Whereas most of hydrolytic peptides have a MW below 2000 Da, preliminary investigations of antimicrobial properties revealed three antibacterial fractions that demonstrate functional activities. The abundance of small peptides as well as the biological activities detected could imply very interesting applications for shrimp hydrolysate in the field of aquaculture feeding.

DOI10.1016/j.jbiotec.2014.06.020
Alternate JournalJ. Biotechnol.
Identifiant (ID) PubMed24998765