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Comparative effects of a non-steroidal ecdysone agonist RH-5992 and 20-hydroxyeedysone in a lepidopteran cell line (IAL-PID2)

TitreComparative effects of a non-steroidal ecdysone agonist RH-5992 and 20-hydroxyeedysone in a lepidopteran cell line (IAL-PID2)
Type de publicationJournal Article
Year of Publication2005
AuteursAuzoux-Bordenave, S, Solvar, M, Queguiner, I, Bozzolan, F, Mottier, V, Siaussat, D, Porcheron, P, Debernard, S
JournalInsect Biochemistry and Molecular Biology
Volume35
Pagination1033–1042
ISSN0965-1748
Résumé

The non-steroidal ecdysone agonist, RH-5992, exhibits ecdysteroid activities in vivo as well as in vitro more effectively than 20-hydroxyecdysone (20E). Using the IAL-PID2 cells derived from imaginal wing discs of last larval instar of Plodia interpunctella, we investigated the action of RH-5992 in the control of cell growth. Its effects on the proliferative activity of IAL-PID2 cells, the induction level in G2/M arrest and on the expression rate of Plodia B cyclin (PcycB), ecdysone B1-isoform (PIEcR-B1) and Ultraspiracle-2 isoform (PIUSP-2) were examined. From these cellular and molecular assays, our results brought evidence that RH-5992, like 20E, induced an inhibition oil cell proliferation by blocking IAL-PID2 cells in G2/M phase. Moreover, this G2/M arrest was preceded by a decrease in the expression level of PcycB and a high induction of PIEcR-B1, PIUSP-2 mRNAs. Dose-response experiments revealed that RH-5992 was even more potent than 20E. On these parameters, we therefore suggest that the differential observed in the expression level of USP and EcR by RH-5992 and 20E could contribute to the difference observed for the biological potency of these two compounds. (C) 2005 Elsevier Ltd. All rights reserved.

DOI10.1016/j.ibmb.2005.04.004