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A complex set of sex pheromones identified in the cuttlefish Sepia officinalis.

TitreA complex set of sex pheromones identified in the cuttlefish Sepia officinalis.
Type de publicationJournal Article
Year of Publication2012
AuteursEnault, J, Zatylny-Gaudin, C, Bernay, B, Lefranc, B, Leprince, J, Baudy-Floc'h, M, Henry, J
JournalPLoS One
Volume7
Ticket10
Paginatione46531
Date Published2012
ISSN1932-6203
Mots-clésAmino Acid Sequence, Animal Migration, Animals, Decapodiformes, Expressed Sequence Tags, Female, Gene Expression Profiling, Gene Library, Mass Spectrometry, Molecular Sequence Data, Peptides, Reproduction, Seasons, Sequence Analysis, DNA, Sex Attractants
Résumé

BACKGROUND: The cephalopod mollusk Sepia officinalis can be considered as a relevant model for studying reproduction strategies associated to seasonal migrations. Using transcriptomic and peptidomic approaches, we aim to identify peptide sex pheromones that are thought to induce the aggregation of mature cuttlefish in their egg-laying areas.

RESULTS: To facilitate the identification of sex pheromones, 576 5'-expressed sequence tags (ESTs) were sequenced from a single cDNA library generated from accessory sex glands of female cuttlefish. Our analysis yielded 223 unique sequences composed of 186 singletons and 37 contigs. Three major redundant ESTs called SPα, SPα' and SPβ were identified as good candidates for putative sex pheromone transcripts and are part of the 87 unique sequences classified as unknown. The alignment of translated SPα and SPα' revealed a high level of conservation, with 98.4% identity. Translation led to a 248-amino acid precursor containing six peptides with multiple putative disulfide bonds. The alignment of SPα-α' with SPβ revealed a partial structural conservation, with 37.3% identity. Translation of SPβ led to a 252-amino acid precursor containing five peptides. The occurrence of a signal peptide on SPα, SPα' and SPβ showed that the peptides were secreted. RT-PCR and mass spectrometry analyses revealed a co-localization of transcripts and expression products in the oviduct gland. Preliminary in vitro experiments performed on gills and penises revealed target organs involved in mating and ventilation.

CONCLUSIONS: The analysis of the accessory sex gland transcriptome of Sepia officinalis led to the identification of peptidic sex pheromones. Although preliminary functional tests suggested the involvement of the α3 and β2 peptides in ventilation and mating stimulation, further functional investigations will make it possible to identify the complete set of biological activities expected from waterborne pheromones.

DOI10.1371/journal.pone.0046531
Alternate JournalPLoS ONE
Identifiant (ID) PubMed23118854
PubMed Central IDPMC3484142