Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera

TitreIdentification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera
Type de publicationJournal Article
Year of Publication2006
AuteursDuplat, D, Puissegur, M, Bedouet, L, Rousseau, A, Boulzaguet, H, Milet, C, Sellos, D, Van Wormhoudt, A, Lopez, E
JournalFebs Letters
Volume580
Pagination2435–2441
ISSN0014-5793
Résumé

Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95-99% calcium carbonate and 1-5%, organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

DOI10.1016/j.febslet.2006.03.077