|Titre||Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera|
|Type de publication||Journal Article|
|Year of Publication||2006|
|Auteurs||Duplat, D, Puissegur, M, Bedouet, L, Rousseau, A, Boulzaguet, H, Milet, C, Sellos, D, Van Wormhoudt, A, Lopez, E|
Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95-99% calcium carbonate and 1-5%, organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.