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Comparative ultrastructure and carbohydrate composition of gastroliths from astacidae, cambaridae and parastacidae freshwater crayfish (crustacea, decapoda).

TitleComparative ultrastructure and carbohydrate composition of gastroliths from astacidae, cambaridae and parastacidae freshwater crayfish (crustacea, decapoda).
Publication TypeJournal Article
Year of Publication2012
AuthorsLuquet, G, Fernandez, MS, Badou, A, Guichard, N, Le Roy, N, Corneillat, M, Alcaraz, G, Arias, JL
JournalBiomolecules
Volume3
Issue1
Pagination18-38
Date Published2012
ISSN2218-273X
Abstract

Crustaceans have to cyclically replace their rigid exoskeleton in order to grow. Most of them harden this skeleton by a calcification process. Some decapods (land crabs, lobsters and crayfish) elaborate calcium storage structures as a reservoir of calcium ions in their stomach wall, as so-called gastroliths. For a better understanding of the cyclic elaboration of these calcium deposits, we studied the ultrastructure of gastroliths from freshwater crayfish by using a combination of microscopic and physical techniques. Because sugars are also molecules putatively involved in the elaboration process of these biomineralizations, we also determined their carbohydrate composition. This study was performed in a comparative perspective on crayfish species belonging to the infra-order Astacidea (Decapoda, Malacostraca): three species from the Astacoidea superfamily and one species from the Parastacoidea superfamily. We observed that all the gastroliths exhibit a similar dense network of protein-chitin fibers, from macro- to nanoscale, within which calcium is precipitated as amorphous calcium carbonate. Nevertheless, they are not very similar at the molecular level, notably as regards their carbohydrate composition. Besides glucosamine, the basic carbohydrate component of chitin, we evidenced the presence of other sugars, some of which are species-specific like rhamnose and galacturonic acid whereas xylose and mannose could be linked to proteoglycan components.

DOI10.3390/biom3010018
Alternate JournalBiomolecules
PubMed ID24970155
PubMed Central IDPMC4030881