User login

Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).

TitleHemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).
Publication TypeJournal Article
Year of Publication2014
AuthorsLe Pabic, C, Goux, D, Guillamin, M, Safi, G, Lebel, J-M, Koueta-Noussithé, S, Serpentini, A
JournalFish Shellfish Immunol
Volume40
Issue2
Pagination362-73
Date Published2014 Oct
ISSN1095-9947
Abstract

Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.

DOI10.1016/j.fsi.2014.07.020
Alternate JournalFish Shellfish Immunol.
PubMed ID25066968