|Title||Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Le Pabic, C, Goux, D, Guillamin, M, Safi, G, Lebel, J-M, Koueta-Noussithé, S, Serpentini, A|
|Journal||Fish Shellfish Immunol|
|Date Published||2014 Oct|
Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.
|Alternate Journal||Fish Shellfish Immunol.|