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Identification of three singular glycosyl hydrolase family 18 members from the oyster Crassostrea gigas: Structural characterization, phylogenetic analysis and gene expression.

TitleIdentification of three singular glycosyl hydrolase family 18 members from the oyster Crassostrea gigas: Structural characterization, phylogenetic analysis and gene expression.
Publication TypeJournal Article
Year of Publication2011
AuthorsBadariotti, F, Lelong, C, Dubos, M-P, Favrel, P
JournalComp Biochem Physiol B Biochem Mol Biol
Volume158
Issue1
Pagination56-63
Date Published2011 Jan
ISSN1879-1107
KeywordsAnimals, Crassostrea, Gene Expression Regulation, Enzymologic, Glycoside Hydrolases, Phylogeny, Protein Conformation, Species Specificity
Abstract

Glycoside hydrolase family 18 (GH18) includes chitinases and non-enzymatic chitinase-like proteins (CLPs) with representatives among eukaryotes (animals and plants), prokaryotes and viruses. In Lophotrochozoa, one of the three clades of bilaterian animals, only three members (Cg-Clp1, Cg-Clp2 and Cg-Chit) have been reported from the bivalve mollusc Crassostrea gigas. Here, we describe the cloning and the characterization of two additional chitinases (Cg-Chit2 and Cg-Chit3) and a new CLP (Cg-Clp3) from this species. Cg-Chit2 presents an atypical C-terminal hydrophobic region acting probably as a GPI-anchor signal for plasma membrane attachment. On the contrary, Cg-Chit3 displays a C-terminal truncated structure leading to a possible sequestration in lysosomes. Phylogenetic analyses suggest that CLPs have appeared independently in the three main branches of bilaterian animals, as a result of convergent evolution. Gene expression profiles analyzed by quantitative RT-PCR support the involvement of Cg-Clp3 in embryonic development, adult oyster growth and tissue remodelling during metamorphosis and gonadal restructuring.

DOI10.1016/j.cbpb.2010.09.009
Alternate JournalComp. Biochem. Physiol. B, Biochem. Mol. Biol.
PubMed ID20868765