| Title | Molecular and physiological characterization of an invertebrate homologue of a calcitonin-related receptor. |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Dubos, M-P, Badariotti, F, Rodet, F, Lelong, C, Favrel, P |
| Journal | Biochem Biophys Res Commun |
| Volume | 310 |
| Issue | 3 |
| Pagination | 972-8 |
| Date Published | 2003 Oct 24 |
| ISSN | 0006-291X |
| Keywords | Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary, Ions, Molecular Sequence Data, Mollusca, Phylogeny, Receptors, Calcitonin Gene-Related Peptide, Receptors, G-Protein-Coupled, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Sequence Homology, Amino Acid |
| Abstract | Calcitonin is a key hormone involved in the regulation of calcium metabolism in vertebrates. Using oligonucleotide primers derived from consensus sequences of vertebrate calcitonin receptors, we have cloned and characterized the first representative of an invertebrate calcitonin receptor from the bivalve mollusc Crassostrea gigas. This receptor named Cg CT-R exhibits 39% amino acid sequence identity with both human calcitonin and calcitonin gene-related precursor receptors. Cg CT-R is expressed mainly in the gills and the mantle edge as well as at lower levels in muscles, digestive gland, heart, and labial palps. Transfer of animals from seawater to brackish water resulted in a significant decrease of Cg CT-R transcript levels in the gills, thus suggesting a role for ionic balance in molluscs. |
| Alternate Journal | Biochem. Biophys. Res. Commun. |
| PubMed ID | 14550300 |