|Title||Restoration of stratum corneum with nacre lipids|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Rousseau, M, Bedouet, L, Lati, E, Gasser, P, Le Ny, K, Lopez, E|
|Journal||Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology|
To discover potential new products for the atopic dermatitis treatment, lipids extracted from nacre from the oyster Pinctada margaritifera were tested on artificially dehydrated skin explants. Expression of filaggrin and transglutaminase I was investigated after treatment of dehydrated skin with P margaritifera lipid extracts according to light microscopy after labelling with specific monoclonal antibodies. The lipids were extracted from the nacre with methanol/chloroform mixture at room temperature and the extract composition was determined according to TLC and densitometry measures. Relative to the dry nacre material, a yield of extraction in lipids of 0.54% (w/w) was determined. Fatty acids, triglycerides, cholesterol and ceramides were in low abundance. Then, application of lipid formulations on skin explants previously dehydrated gave after 3 h an overexpression of filaggrin and a decrease of transglutaminase expression as shown by light microscopy. Using immunofluorescence labelling, we showed that lipids extracted from the mother of pearl of P. margaritifera induced a reconstitution of the intercellular cement of the stratum corneum. The signaling properties of the nacre lipids could be used for a development of new active product treatment against the symptoms of the dermatitis. (c) 2006 Elsevier Inc. All rights reserved.