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Thermal biology of the deep-sea vent annelid Paralvinella grasslei: in vivo studies.

TitleThermal biology of the deep-sea vent annelid Paralvinella grasslei: in vivo studies.
Publication TypeJournal Article
Year of Publication2008
AuthorsCottin, D, Ravaux, J, Léger, N, Halary, S, Toullec, J-Y, Sarradin, P-M, Gaill, F, Shillito, B
JournalJ Exp Biol
Volume211
IssuePt 14
Pagination2196-204
Date Published2008 Jul
ISSN0022-0949
KeywordsAmino Acid Sequence, Animals, Behavior, Animal, Body Temperature, Hot Temperature, HSP70 Heat-Shock Proteins, Molecular Sequence Data, Oceans and Seas, Oxygen, Phylogeny, Polychaeta, Seawater, Sequence Alignment
Abstract

The annelid Paralvinella grasslei is a deep-sea vent endemic species that colonizes the wall of active chimneys. We report here the first data on its thermal biology based on in vivo experiments in pressurized aquaria. Our results demonstrate that P. grasslei survives a 30 min exposure at 30 degrees C, and suggest that the upper thermal limit of this species is slightly above this temperature. The first signs of stress were noticed at 30 degrees C, such as a significant increase in the animal's activity and the expression of HSP70 stress proteins. A preliminary investigation of the kinetics of stress protein expression surprisingly showed high levels of HSP70 proteins as late as 3.5 h after the heat shock. Finally, we provide here the first sequences for vent annelid hsp70 (P. grasslei, Hesiolyra bergi and Alvinella pompejana). These constitute valuable tools for future studies on the thermal biology of these annelids.

DOI10.1242/jeb.018606
Alternate JournalJ. Exp. Biol.
PubMed ID18587113